2011年7月17日星期日

My Fish

Every time running between QMRI & Chancellor, I was always stopped by questions like ”what’s in your hand ”. So today I am gonna talk about, hush, be quiet, I don’t think my supervisor would like to hear my possessing that personally. Although I haven’t been in charge of any line, I bet everybody would say my dear after seeing them.

Actually these little guys are not easy to deal with. They have three meals per day, twice Artemia, one kind of live bugs, once dry food. We rotate to feed them every day, sometimes even when I am hungry, have to make sure they get food on time first. But watching them chasing Artemia is quite an enjoyable experience. Good Artemia is transparent when splashing into water, at this moment those fish would gather around the food hole, waving their fins to dance with those crystal like Artemia. They also love jumping, seemingly willing to get out of the tanks. But silly kids, don’t they know this would kill themselves?

We tried various ways to make them happy, music, talking or changing water. I ever tried to train them Chinese listening, however, they only turned to be more sensitive to the food. All of these is actually to make them lay more eggs for the experimental use. Sometimes it works, others may not. In that case we have to create as many one night loves as possible to push them. A little bit cruel, isn’t it? But among them, I also found a super couple who can lay tons of high quality eggs every week, which I believe there is definite some true love between them. See, in animal world it proves again true love is unusual stuff so once you come across, just hold it as tightly as possible.

Our fish are different in they can express fluorescent proteins. So under the microscope, it appears to be a distinct world.

2011年6月11日星期六

Recent

These days, so many things occurred, maybe only a mosaic of short stories can cover most of them.

I know I rarely have good luck, but this time it appears even worse. Whenever my determined to do anything, so many unexpected things just pop up to challenge my “tolerance level”.

When preparing to cook a big meal for my lab colleagues last Thursday, I happened to know getting extra work to do, fixing the embryos, which means I need first move three embryos from one well onto a new petri dish, dechorionating them and deyolking, then transfer them into a 24-well plate, add the fixing solution after removing as much water as possible. If one round takes me about 20 minutes at least, not including the interval of cleaning the dish, then repeating almost 30 wells equals 10 hours and it’d been near 6pm at that moment. There is a desperate voice speaking by my side, give up bcz you have more stuff to deal with. Zhen said she felt I almost burst into tears at that moment. Thank goodness for her brilliant advice, putting all the embryos on the lid of the plate so that I can dechorionate and deyolk all of them at the same time, which saves me tremendous time and efforts. Although that night I worked till nine, rushed to the shop for those raw materials and cooked till midnight, I was still proud of that cramped evening. After all I finished everything necessary. Zhen also inspires me that never give up till the last minute and proper arrangement can have astonishing overall improvement of efficiency.

Sometimes the more eagerly you rush to one thing, the more creepy it turns out to be. I spent the entire afternoon to fix a plate of embryos but dropped it eventually when moving the roller into the cold room. How I wish the time could go back again when I would have definitely first placed the plate on the bench then moved the roller! Right now only one big lesson can be obtained from that.

The frustrating result of last project also came out last week. Though I thought to have tried my best last time, it seems there are still more problems to correct. No wonder they say we are all on the journey of learning. Fortunately I got some helping hands.

I think the essence of life is to carry on with various defects. So just be patient.